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Fermentation

Spontaneous (Wild/Native) Fermentation

Technical analysis of spontaneous fermentation using indigenous yeasts; microbial ecology, fermentation dynamics, risk management, and the stylistic and terroir expression implications.

Spontaneous (Wild/Native) Fermentation

Problem Definition

Spontaneous fermentation—relying on indigenous yeasts rather than inoculating with commercial strains—is both the original winemaking method and a contemporary premium technique. Advocates argue for enhanced complexity, terroir expression, and authenticity; critics cite unpredictability, longer fermentation times, and risk of off-flavors or stuck fermentation. Understanding the microbial succession, fermentation dynamics, and risk factors enables informed decisions about when spontaneous fermentation adds value.

Technical Context

Yeast Populations

Indigenous Yeast Sources:

  • Vineyard: Grape skins, leaves, soil
  • Winery: Equipment, surfaces, barrels
  • Air: Ambient microflora

Typical Species Succession:

StageDominant SpeciesCharacteristics
Early (0-3% ABV)Kloeckera/HanseniasporaLow alcohol tolerance; volatile
Mid (3-6% ABV)Metschnikowia, CandidaModerate tolerance
Late (>6% ABV)Saccharomyces cerevisiaeCompletes fermentation

Non-Saccharomyces Contributions

Positive Contributions:

  • Aromatic complexity (esters, thiols)
  • Glycerol production (mouthfeel)
  • β-glucosidase activity (aroma release)
  • Unique metabolic products

Negative Contributions:

  • Acetic acid (volatile acidity)
  • Ethyl acetate (nail polish)
  • Hydrogen sulfide (reduction)
  • Slow/stuck fermentation potential

Fermentation Dynamics

Typical Progression:

  1. Lag phase (1-5 days): Non-Saccharomyces dominate
  2. Exponential phase: Saccharomyces emerges
  3. Stationary phase: Saccharomyces dominates
  4. Completion: Native S. cerevisiae finishes

Duration: Often 2-4× longer than inoculated fermentation

Options and Interventions

Full Spontaneous Fermentation

Protocol:

  1. No commercial yeast addition
  2. No SO₂ at crush (or minimal)
  3. Allow indigenous microflora to develop
  4. Natural succession proceeds

Conditions for Success:

  • Healthy fruit (low spoilage organisms)
  • Winery with established beneficial microflora
  • Experience with technique
  • Tolerance for variation

Pied de Cuve (Starter)

Concept:

  • Pre-fermentation indigenous yeast culture
  • Made from own grapes
  • Started before main harvest
  • Used to inoculate larger volumes

Protocol:

  1. Harvest small quantity early
  2. Crush and allow spontaneous fermentation
  3. When active, use to inoculate main ferment
  4. Speeds initiation while maintaining indigenous character

Advantages:

  • Faster fermentation start
  • Indigenous yeast amplified
  • More predictable than full spontaneous
  • Traditional technique

Hybrid Approaches

Spontaneous Start, Inoculated Finish:

  • Allow spontaneous fermentation to begin
  • Inoculate at 5-8% potential alcohol if stuck
  • Captures non-Saccharomyces complexity
  • Safety net for completion

Co-Inoculation:

  • Commercial non-Saccharomyces strain
  • Followed by Saccharomyces inoculation
  • Controlled “spontaneous” character
  • Reproducible results

Trade-offs and Risks

Extended Fermentation Time

Typical Duration:

  • Spontaneous: 3-8 weeks (or more)
  • Inoculated: 1-3 weeks

Implications:

Stuck/Sluggish Fermentation

Risk Factors:

  • Low nutrient levels
  • Temperature fluctuations
  • Insufficient Saccharomyces population
  • High initial sugar

Mitigation:

  • Nutrient monitoring/supplementation
  • Temperature control
  • Pied de cuve technique
  • Backup inoculation plan

Off-Flavor Development

Risks:

Mitigation:

  • Healthy fruit
  • Temperature control
  • Monitor VA regularly
  • Intervention if needed

Unpredictability

Vintage Variation:

  • Microbial populations vary
  • Different fermentation character each year
  • Can be feature (complexity) or bug (inconsistency)

Practical Implications

Winery Microflora Development

Building Beneficial Population:

  • Consistent spontaneous fermentation builds population
  • “House character” develops over years
  • Barrel microflora important
  • Sanitation balance critical

New Wineries:

  • May lack established beneficial yeasts
  • Higher risk initially
  • Consider hybrid approaches
  • Patience required

Regional/Producer Examples

Burgundy:

  • Traditional spontaneous fermentation
  • Cellar microflora prized
  • Chardonnay and Pinot Noir
  • Premium producers often spontaneous

Barolo:

  • Traditional spontaneous
  • Nebbiolo extended fermentation
  • Some modernists inoculate
  • Traditional producers: indigenous only

Natural Wine Movement:

  • Spontaneous fermentation central
  • Minimal intervention philosophy
  • Variable quality
  • Philosophical commitment

Decision Framework

FactorFavors SpontaneousFavors Inoculation
Fruit healthExcellentCompromised
Winery historyEstablished microfloraNew/sterile
Style goalComplexity, terroirConsistency, fruit
Risk toleranceHighLow
ExperienceExperiencedNovice
MarketPremium, nicheCommercial

Monitoring Requirements

Critical Measurements:

  • Temperature (more critical—longer fermentation)
  • Sugar (Brix/density) daily
  • Volatile acidity (weekly)
  • Sensory evaluation (daily)
  • Microscopy (optional—yeast population)

References

  • Fleet, G.H. (2003). “Yeast interactions and wine flavor.” International Journal of Food Microbiology, 86, 11-22. DOI: 10.1016/S0168-1605(03)00245-9

  • Pretorius, I.S. (2000). “Tailoring wine yeast for the new millennium.” Yeast, 16, 675-729. DOI: 10.1002/1097-0061(20000615)

  • Jolly, N.P., Varela, C., & Pretorius, I.S. (2014). “Not your ordinary yeast: Non-Saccharomyces yeasts in wine production uncovered.” FEMS Yeast Research, 14, 215-237. DOI: 10.1111/1567-1364.12111

  • Combina, M., et al. (2005). “Dynamics of indigenous yeast populations during spontaneous fermentation.” International Journal of Food Microbiology, 99, 237-243. DOI: 10.1016/j.ijfoodmicro.2004.08.017


Last Updated: January 6, 2026